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KMID : 0923620090090060265
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Immune Network
2009 Volume.9 No. 6 p.265 ~ p.273
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Recombinant DNA and Protein Vaccines for Foot-and-mouth Disease Induce Humoral and Cellular Immune Responses in Mice
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Bae Ji-Young
Moon Sun-Hwa
Choi Jung-Ah
Kwon Dae-Hyuck
Lee Suk-Chan
Yang Joo-Sung
Park Jong-Sug
Kim Ki-Yong
Kim Byung-Han
Song Jae-Young
Kim Jong-Bum
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Abstract
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Background: Foot-and-mouth disease virus (FMDV) is a small single-stranded RNA virus which belongs to the family Picornaviridae, genus Apthovirus. It is a principal cause of FMD which is highly contagious in livestock. In a wild type virus infection, infected animals usually elicit antibodies against structural and non-structural protein of FMDV. A structural protein, VP1, is involved in neutralization of virus particle, and has both B and T cell epitopes. A RNA-dependent RNA polymerase, 3D, is highly conserved among other serotypes and strongly immunogenic, therefore, we selected VP1 and 3D as vaccine targets.
Methods: VP1 and 3D genes were codon-optimized to enhance protein expression level and cloned into mammalian expression vector. To produce recombinant protein, VP1 and 3D genes were also cloned into pET vector. The VP1 and 3D DNA or proteins were co-immunized into 5 weeks old BALB/C mice.
Results: Antigen-specific serum antibody (Ab) responses were detected by Ab ELISA. Cellular immune response against VP1 and 3D was confirmed by ELISpot assay.
Conclusion: The results showed that all DNA- and protein-immunized groups induced cellular immune responses, suggesting that both DNA and recombinant protein vaccine administration efficiently induced Ag-specific humoral and cellular immune responses.
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KEYWORD
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FMDV, DNA vaccine, Recombinant protein vaccine, B cell epitope peptide
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